花生四烯酸代谢物在湿热型溃疡性结肠炎大鼠的表达与调节

Arachidonic Acid Metabolites Increased in Ulcerative Colitis Rats with Damp-heat Syndrome and were Down-regulated by Huanqin Decoction

目的:探讨花生四烯酸(AA)代谢物在湿热型溃疡性结肠炎(UC)大鼠发病的作用以及黄芩汤的调节。方法:采用高脂高糖饲料结合人工气候箱法造成大鼠湿热,使用三硝基苯磺酸灌肠造成湿热型UC模型;将造模大鼠分为模型组(n=12)、黄芩汤治疗组(n=13)及美沙拉嗪组(n=12)。治疗两周后用ELISA法检测大鼠前列腺素E2(PGE2)、血小板活化因子(PAF)、白三烯B4(LTB4)及环氧合酶-2(COX-2)含量;检测甘油三酯(TG)及总胆固醇(CH)含量并计算粪便脂肪球数量从而反映湿热程度;对结肠进行肉眼组织学评分从而评价UC的严重程度;将AA代谢物与以上湿热型UC指标进行相关性分析。结果:与空白组(n=12)比较,造模组血清及结肠PGE2、PAF、LTB4及COX-2含量明显升高(P<0.05);治疗后黄芩汤组的PGE2及LTB4均显著低于美沙拉嗪组(P<0.05),但PAF及COX-2无组间差异。湿热指标方面,治疗后黄芩汤组血脂及粪便脂肪球数量均显著低于美沙拉嗪组(P<0.05);UC方面,黄芩汤及美沙拉嗪组的组织学评分无统计学差异。相关性分析显示组织评分与AA代谢物、血脂及粪便脂肪球均呈显著正相关性(r>0.930,P<0.01)。结论:AA代谢物在湿热型UC大鼠血清及结肠明显升高,且与反映湿热的血脂及粪便脂肪球、与反映UC程度的组织学评分均呈正相关性;黄芩汤对代谢物PGE2、LTB4及对湿热指标的调节作用优于美沙拉嗪。

Objective：To explore the role of arachidonic acid metabolites（AAMs） in ulcerative colitis（UC） rats with damp-heat syndrome（DHS）,and to observe the effect of Huanqin decoction on the model.Methods：45 rats were put into an artificial bioclimatic chamber with temperature of 36℃,humidity of 98% and feed in fat and sugar forage to establish the model for 4 weeks.After a one-week treatment by Huanqin decoction（group H,n=13） and by mesalazine（group M,n=12）,concentration of PGE2,LTB4,PAF and COX-2 in serum and colon were detected using ELISA.Triglycercide（TG） and T-cholesterol（CH） were also tested to reflect the degree of DHS,so were the fat globules in the feces.Histological scores for colon were rated through naked eyes to assess the severity of UC.Besides,the relations between the upper indexes were calculated by Pearson analysis.Results：Compared with the control group（n=15）,PGE2,PAF,LTB4 and COX-2 increased significantly in the model rats（n=12,P〈0.05）.PGE2 and LTB4 in group H were obviously lower than that in group M,so were the TG,CH and feces fat globules（all P〈0.05）.But there was no statistical difference of the histological scores between group H and M.Pearson analysis showed there was a definited positive relationship between any two of PGE2,PAF,LTB4,COX-2,TG,CH,feces fat globules and histological scores（r〉0.930,P〈0.01）.Conclusion：AAMs increased apparently in UC rats with DHS,which was closely related to the indexes involving both UC and damp-heat syndrome.It was proved to be more effective of Huangqin decoction to down-regulate PGE2,LTB4,TG,CH and and feces fat globules than mesalazine.