仔猪渗出性皮炎病原的分离鉴定及耐药性分析

Isolation,Identification and Antimicrobial Susceptibilities of Pathogens of Exudative Epidermitis

为消除渗出性皮炎对仔猪的危害,对发生该病的病死仔猪进行细菌分离培养。通过革兰氏染色、生化试验与16SrDNA基因序列同源性分析鉴定:分离出的两株细菌为猪葡萄球菌和松鼠葡萄球菌。猪葡萄球菌和松鼠葡萄球菌能分别产生表皮脱落毒素ExhB和ExhC,表明这两株葡萄球菌都有致病力。猪葡萄球菌和松鼠葡萄球菌均对头孢菌素Ⅰ类(先锋霉素Ⅰ、Ⅴ、Ⅵ)、头孢噻肟和丁胺卡那敏感,在临床上可选择这些药物预防和治疗该病。

To eliminate harm of exudative epidermitis（EE） to piglets,pathogens of EE were isolated and studied.Two gram positive staphylococcus strains were isolated from sick piglets suffered EE.The two isolates were identified as staphylococcus hyicus and staphylococcus sciuri by biochemistry test and analyzing the sequence of 16S rDNA gene.S.hyicus can produce exfoliative toxin ExhB,while S.sciuri can produce exfoliative toxin ExhC,which indicates the two isolates are pathogenic.The two isolates are sensitive to cefalotin,cephazolin,cephradine,cefotaxime and amikacin which shows sick piglets can be treated with these antibiotics.