摘要
目的确定发芽大豆中异黄酮的最佳富集条件,并分析其组成特征。方法用紫外分光光度法测定大豆异黄酮含量;分析固定适宜的芽长条件,以浸泡时间、培养温度及培养湿度3个因素,设计3个水平进行正交试验,确定最佳富集条件;用高效液相色谱法分析最佳富集条件下大豆异黄酮的组成特征。结果发芽大豆芽长30 mm时,异黄酮最佳富集条件为浸泡时间16 h,培养温度25℃,培养湿度90%;最佳富集条件下大豆异黄酮主要由金雀异黄酮、黄豆苷元与黄豆黄素等单体成分组成。结论大豆通过优化发芽培养条件可以富集异黄酮,尤其可提高功能因子金雀异黄酮与黄豆苷元等单体含量,为开发富含大豆异黄酮的功能性大豆食品提供了一定的理论指导。
Objective To determine the optimum conditions for enrichment of soybean isoflavones in germinated soybean and analyze its composing characteristics. Methods The content of soybean isoflavones was determined by UV spectrophotometry. After the suitable length of germinated soybean was fixed, in order to determine the optimal enrichment conditions, the effects of soaking time, culture temperature and humidity on the content of soybean isoflavones were researched by a L9(34 ) orthogonal test. Then the composing characteristics of isoflavones in germinated soybean obtained under the optimal enrichment conditions were analyzed by HPLC method. Results The optimal enrichment conditions of soybean isoflavones were identified as soaking time of 16 h, culture temperature of 25~C, culture humidity of 90% when the length of germinated soybean was 30mm. Under these conditions, the isoflavones primarily consisted of three monomers which were genistein, daidzein and glycitein. Conclusion The optimization of culture conditions of germinated soybean can enrich isoflavones, especially increasing the contents of function factors such as genistein and daidzein, which provides some theoretical guidance for the further development of functional, isoflavone-rich soybean food.
出处
《食品与药品》
CAS
2011年第1期26-30,共5页
Food and Drug
基金
上海市科委(10DZ0504000
1052NM06700)资助
上海交通大学青年基金(AB150200)资助
关键词
发芽
大豆异黄酮
富集
组成特征
germination
soybean isoflavone
enrichment
composing characteristics