期刊文献+

Notch1受体在乳腺癌组织中的表达及临床意义的研究 被引量:1

Expression of Notch1 in Human Breast Cancer Tissue and Its Clinical Significance
下载PDF
导出
摘要 目的探讨乳腺癌组织中Notch1受体的表达水平,为乳腺癌的防治提供新的策略。方法进行荧光定量PCR(FQ-PCR)和免疫组化技术对60例乳腺癌组织和25例正常乳腺组织中的Notch1受体蛋白及基因表达情况的检测分析。结果 (1)FQ-PCR技术检测显示乳腺癌患者Notch1基因表达阳性,DNA拷贝数显著高于对照组,差异有显著性(P<0.05);侧腋窝淋巴结转移组Notch1 DNA拷贝数稍高于非转移组,但差异无显著性(P>0.05)。(2)免疫组织化学染色的结果表明Notch1分子在乳腺癌组织中呈强表达,正常乳腺组织中不表达或弱表达,差异有显著性(P<0.05)。结论 Notch1信号在乳腺上皮干细胞恶性转化中表达上调,Notch1基因可能是一种癌基因,对乳腺癌干细胞的分化起调节作用。 Objective To investigate the role of Notch1 receptor in human breast carcinoma tissue in the prevention and treatment of liver cancer. Methods Sixty cases of invasive ductal carcinoma were collected in this study and RT-PCR and immunohistochemistry methods were used to detect the expression level of Notchl receptor in human breast carcinoma and normal breast tissue, followed by statistical analysis. Results The gene expression of Notchl was significantly up-regulated in human breast carcinoma (P〈0.05). The result of immunohistochemical staining was similar to that of the RT-PCR,and the DNA copy number of Notchl in the lateral axillary lymph node metastasis was slightly higher than that of non-metastasis,with no significant difference(P〉0.05). Conclusion During the malignant transformation of mammary stem cells,the Notchl expression is up-regulated. Notchl gene may be an oncogene candidate, which may regulate the differentiation of mammary cancer stem cells.
出处 《中国现代医生》 2011年第1期1-2,5,共3页 China Modern Doctor
关键词 乳腺癌 NOTCH1 基因表达 免疫组化染色 Breast carcinoma Notch 1 Gene expression Immunohistochemical staining
  • 相关文献

参考文献9

  • 1邵军,潘翠萍,马彪.乳腺干细胞的信号转导与乳腺癌[J].肿瘤,2007,27(9):760-762. 被引量:6
  • 2Allman D,Punt JA,Izon DJ,et al. An invitation to T and more:notch signaling in lymphopoiesis[J]. Cell, 2002,109: S1 -S11.
  • 3Radtke F,Raj K,Kenneth. The role of Notch in tumorigenesis: oncogene or tumour suppressor[J]. Nat Rev Cancer,2003,3(10):756-767.
  • 4Gallahan D,Kozak C,Callahan R. A new common integration region (int-3)for mouse mammary tumor virus on mouse chromosome17[J]. J Virol, 1987,61 ( 1 ) : 218-220.
  • 5Zagouras P,Stifani S,Blaumueller CM. Artavanis-Tsakonas S. Alterations in Notch signaling in neoplastic lesions of the human cervix[J]. Proc Nat Acad Sci USA, 1995,92(14) :6414-6418.
  • 6Talora C,Sgroi DC,Crum CP,et al. Specific down-modulation of Notchl signaling in cervical cancer cells is required for sustained HPV-E6/E7 expression and late steps of malignant transformation[J]. Genes Dev,2002,16(17) :2252-2263.
  • 7李大卫,吴晴,彭志海,杨兆瑞,王一.组织芯片研究Notch1与NF-κB在胃癌中的表达和意义[J].肿瘤,2007,27(6):458-461. 被引量:23
  • 8鲁照明,刘红涛,许培荣,侯桂琴,薛乐勋.Notch1信号途径在食管鳞癌细胞株EC9706的作用研究[J].中国肿瘤临床,2007,34(13):740-743. 被引量:11
  • 9Dontu G,Jackson KW,Kawamura MJ,et al. Role of Notch signaling in cell-fate determination of human mammary stem/progenitor cells[J]. Breast Cancer Res,2004,6(6):605-615.

二级参考文献52

  • 1田芳,侯卫红,许培荣,王建民,陈华艳,薛乐勋,陈奎生.食管鳞癌细胞中NF-κB与IκBα的mRNA、蛋白表达[J].中国肿瘤临床,2006,33(4):198-201. 被引量:7
  • 2LEONG K G,KARSAN A.Recent insights into the role of Notch signaling in tumorigenesis[J].Blood,2006,107(6):2223-2233.
  • 3LOERCHER A,LEE T L,RICKER J L,et al.Nuclear factorkappaB is an important modulator of the altered gene expression profile and malignant phenotype in squamous cell carcinoma[J].Caricer Res,2004,64(18):6511-6523.
  • 4MASSI D,TARANTINI F,FRANCHI A,et al.Evidence for differential expression of Notch receptors and their ligands in melanocytic nevi and cutaneous malignant melanoma[J].Mod Pathol,2006,19(2):246-254.
  • 5STYLIANOU S,CLARKE RB,BRENNAN K.Aberrant activation of Notch signaling in human breast cancer[J].Cancer Res,2006,66(3):1517-1525.
  • 6FAN X,MATSUI W,KHAKI L,et al.Notch pathway inhibition depletes stem-like cells and blocks engraftment in embryonal brain tumors[J].Cancer Res,2006,66(15):7445-7452.
  • 7Katoh M,Katoh M.Notch signaling in gastrointestinal tract[J].Int J Oncol,2007,30(1):247-251.
  • 8SASAKI N,MORISAKI T,HASHIZUME K,et al.Nuclear factorkappaB p65(RelA)transcription factor is constitutively activated in human gastric carcinoma tissue[J].Clin Cancer Res,2001,7(12):4136-4142.
  • 9VILIMAS T,MASCARENHAS J,PALOMERO T,et al.Targering the NF-kappaB signaling pathway in Notchl-induced T-cell leukemia[J].Nat Med,2007,13(1):70-77.
  • 10RAMDASS B,MALIEKAL TT,LAKSHMI S,et al.Coexpression of Notchl and NF-kappaB signaling pathway components in human cervical cancer progression[J].Gynecol Oncol,2007,104(2):352-361.

共引文献34

同被引文献9

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部