189株嗜麦芽寡养单胞菌耐药性检测与氨基糖苷类修饰基因的分布

Detection of drug resistance of 189 strains of stenotrophomonas maltophilia and distribution of aminoglycoside modifying enzymes genes

目的调查嗜麦芽寡养单胞菌对常用抗菌药物的耐药率及氨基糖苷类修饰基因的分布状况。方法采用琼脂扩散试验法,对临床分离的189株嗜麦芽寡养单胞菌的耐药性进行了检测;以PCR法检测189株嗜麦芽寡养单胞菌氨基糖苷类修饰酶基因。结果临床分离的189株嗜麦芽寡养单胞菌对亚胺培南和阿米卡星的耐药率分别为19.05%和23.81%;其中,aac(3)-Ⅰ检测到23株、aac(3)-Ⅱ检测到45株、aac(6)-Ⅰb检测到13株、aac(6)-Ⅱ检测到9株,分别占12.17%、23.81%、6.88%、4.76%。结论嗜麦芽寡养单胞菌对临床常用抗菌药物耐药性已非常严重,存在大量耐药基因。

OBJECTIVE To investigate the drug resistance rate of Stenotrophomonas maltophilia.and the distribution of aminoglycoside modifying enzymes genes in hospital.METHODS A total of 189 strains of S.maltophilia were isolated from clinical samples.The antibiotics susceptibility was performed by using the K-B method.The genes of aminoglycoside modifying enzyme were detected by polymerase chain reaction（PCR）.RESULTS Among the 189 strains of S.maltophilia,the drug resistant rates to imipenem and amikacin were 19.05% and 23.81%,respectively.In addition,there were 23 strains（12.17%）,45 strains（23.81%）,13 strains（6.88%） and 9 strains（4.76%） carring the gene of aac（3）-Ⅰ,aac（3）-Ⅱ,aac（6）-Ⅰb and aac（6）-Ⅱ,respectively.CONCLUSION The drug resistance of S.maltophilia to commonly used antibiotics were very serious,in addition,S.maltophilia strains often have lots of drug resistant genes.