腹泻患儿轮状病毒感染G、P基因型分析

Genotypes G and P of rotavirus causing infection in diarrhea children

目的研究急性腹泻患儿A群轮状病毒感染的流行情况。方法采用ELISA试剂盒检测筛选轮状病毒抗原阳性标本,收集并进行PCR检测VP4、VP7型别。结果经ELISA试剂盒检测,350份标本中检出126份轮状病毒抗原阳性,检出率为36.00%,G分型以G3型为主(63.49%),其次为G1型(19.05%),P分型以P8型为主(61.11%),其次为P4型(18.25%)。结论轮状病毒是儿童急性腹泻的主要病原体之一,G3、P8为主要流行株。

OBJECTIVE To investigate the epidemic state of group A rotavirus causing infection in acute diarrhea children.METHODS The positive samples of antigens of rotavirus were detected and screened out by using ELISA,and VP4 and VP4 were detected by PCR.RESULTS A total of 126 cases of positive antigens of rotavirus were detected from 350 cases of samples with the detection rate of 36.00%,G3（63.49%） was the major G type,the following was G1（19.05%）,and P8（61.11%） was the major P type,the following was P4（18.25%）.CONCLUSION The infection of rotavirus is the major cause of children acute diarrhea disease.Genotype of G3 and P8 are the most prevalent stains.