摘要
采用多糖衍生物手性色谱柱,建立了正相高效液相色谱法(NP-HPLC)拆分达非那新中间体对映体。考察了该对映体在不同手性固定相上的分离趋势及保留顺序,以及流动相组成对该中间体对映体的分离度和洗脱顺序的影响。结果表明:用Chiralpak AD和Chiralcel OJ柱均能实现对映体的有效分离,为达非那新中间体的光学纯度检测提供了可靠的分离方法。
A NP-HPLC method was established for the separation of darifenacin intermediate enantiomers with polysaccharide chiral column.Enantiomeric separation trend and retention order on different chiral stationary phases were investigated,as well as the separation and elution order by influence of mobile phase composition.The effective separation of enantiomers was obtained with Chiralpak AD and Chiralcel OJ column,which provided a reliable separation method for the optical purity determination of darifenacin intermediate.
出处
《光谱实验室》
CAS
CSCD
北大核心
2011年第1期188-191,共4页
Chinese Journal of Spectroscopy Laboratory
关键词
达非那新中间体
手性固定相
对映体
分离
Darifenacin Intermediate
Chiral Stationary Phase
Enantiomers
Separation
