CPA矩阵分光光度法同时测定苯二酚异构体

Simultaneous Determination of Isomers of Dihydroxybenzenes by Spectrophotometry with CPA Matrix

对吸收峰严重重叠的邻苯二酚、间苯二酚和对苯二酚3组份体系同时测定进行了研究。提出了3波长CPA矩阵分光光度法,即以283.5、279.5、276.5nm3波长为3种苯二酚的测量波长,按正交设计表L25(56)配制25组标准混合溶液,对3种苯二酚进行校正;同时测定了模拟混合样本中的邻苯二酚、间苯二酚和对苯二酚含量。测量结果表明,邻苯二酚在浓度8μg/mL时误差稍大,绝大部分分析结果相对误差小于5%,测量体系稳定,结果可靠。

Simultaneous determination of pyrocatechol,resorcinol and hydroquinone was studied by spectrophotometry.The absorption spectra of these three components were serious overlap in ultraviolet spectral range.The spectrophotometry with three wavelengths CPA matrix was proposed.Three wavelengths at 283.5,279.5,276.5nm were selected as determination wavelengths.25 mixture standard resolutions were prepared as orthogonal projection form L25（56）.Three kinds of the components were corrected.The contents of pyrocatechol,resorcinol and hydroquinone in simulation mixture samples were measured,simultaneously.The relative error of pyrocatechol was a bit large when the concentration was 8μg/mL,and most of the relative error was less than 5%.The measurement system is stable and reliable.