CXCL16基因多态性与C反应蛋白的相关性研究

Research on the association between CXCL16 gene polymorphism and C reactive protein levels

目的探讨健康人群趋化因子CXC配体16（CXCL16）基因多态性与血清CXCL16、C反应蛋白（CRP）水平的相关性。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测204名健康体检者CXCL16基因A181V多态性,同时分别应用酶联免疫吸附试验（ELISA）和免疫比浊法检测血清CXCL16和高敏CRP（hs-CRP）水平,并进行统计学分析。结果 AA基因型血清hs-CRP水平明显高于GG、GA基因型（P=0.01）;多元线性回归显示A等位基因是高hs-CRP水平的独立危险因素（OR=1.25,95%CI：1.08~2.59,P=0.021）;以血清hs-CRP水平中位数（0.91 mg/L）为界定值分为2组,AA基因型和A等位基因频率在hs-CRP〉0.91 mg/L组中显著升高（P=0.008,0.036）;而血清CXCL16水平与其基因型间差异无统计学意义（P〉0.05）。结论 CXCL16基因A181V位点基因多态性与血清hs-CRP水平相关,A等位基因可能参与机体炎症反应的发生、发展。

Objective To investigate the association of serum chemokine CXC ligand 16（CXCL16）and C reactive protein（CRP） levels with CXCL16 gene polymorphism in healthy subjects.Methods The polymorphisms of CXCL16 gene A181V were detected in 204 healthy subjects by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）.Enzyme-linked immunosorbent assay（ELISA） and particle-enhanced immunonephelometry were used to detect serum CXCL16 and high sensitive CRP（hs-CRP） levels respectively,and the results were analyzed statistically.Results Serum hs-CRP levels in AA genotype were significantly higher than those in GG and GA genotypes（P=0.01）.Multiple linear regression showed that A allele was an independent risk factor of high-level hs-CRP（OR=1.25,95%CI：1.08-2.59,P=0.021）.2 groups were set according to the median of hs-CRP levels（0.91 mg/L）.AA genotype and A allele frequencies were significantly higher in group with higher hs-CRP level than those in group with lower one（P=0.008,0.036）.However,there was no significant difference between serum CXCL16 levels and its genotypes（P〈0.05）.Conclusions CXCL16 gene A181V polymorphism is associated to serum hs-CRP levels,and A allele may be involved in the occurrence and development of inflammatory response.