红花黄色素对内皮细胞增殖及凋亡的保护作用

Protection effects of hydroxysafflor yellow A on proliferation and apoptosis of human umbilicalvein endothelial cells

目的通过LPC诱导建立体外再狭窄内皮细胞模型并探讨红花黄色素对对血管内皮细胞的保护作用。方法体外培养人脐静脉内皮细胞(HUVECs),分为:(1)正常对照组,(2)溶血卵磷脂(LPC)组,(3)低浓度红花黄色素组(50 mg/L),(4)高浓度红花黄色素组组(100 mg/L)。分别观测内皮细胞增殖、凋亡及上清液一氧化氮(NO)浓度的的变化。结果与正常对照组比较,LPC抑制内皮细胞增殖,促进细胞凋亡,降低NO浓度。红花黄色素干预LPC对内皮细胞的作用,使内皮细胞增殖增强,细胞凋亡减少,NO浓度升高。结论红花黄色素可改善LPC对HUVECs的影响,使内皮细胞增殖增强,凋亡减少,升高NO浓度。

Objective To establish the cell model of restenosis in vitro induced by LPC and investi-gate the effects of HYSA on the protection and treatment of the restenosis1.Methods Human umbilical vein endothelial cells（HUVECs） were cultured in vitro.The study was designated to 4 groups：（1） control group,（2）LPC group,（3）Low-concentration HYSA（50 mg/L） group,（4）high-concentration HYSA（100 mg/L） group.Proliferation and apoptosis of HUVECs were assessed by MTT assay,flow cytometry（FCM） and electron microscope.The nitricoxide（NO） concentration were determned by nitrate reductase assay.respectively.Results Compared with the control group,LPC could inhibit the growth and induce apoptosis of HUVECs as well as decrease NO production in HUVECs.HYSA could increase the proliferation and decrease the apoptosis of HUVECs,and also enhance NO production of HUVECs which induced by LPC.Conclusion HYSA could improve proliferation and reduce apoptosis and enhance NO production of HUVECs through lysophosphatidylcholine,which may be an important route for HYSA to prevent and treat the restenosis after percutaneous coronary intervention.