摘要
目的评价巢式PCR在烟曲霉侵袭性感染早期病原学诊断中的应用价值。方法构建烟曲霉播散性感染动物模型,收集不同感染时段动物血液标本20份,分别进行巢式PCR检测和真菌培养,并对感染动物多次进行影像学检查;测定25例临床诊断为侵袭性曲霉病患者的45份血清样本,并与血清半乳甘露聚糖(GM)测定结果进行比较。结果巢式PCR检测疾病模型动物血清曲霉特异基因,标本阳性率为80%(16/20),最早在感染后的第二天即可检出;而在整个感染过程中,血培养结果均为阴性,影像学检查则在感染后期才提示真菌感染的存在。临床标本的检测结果显示,巢式PCR敏感性和特异度分别为64%和100%。结论巢式PCR具有较好的敏感性及特异度,可用于曲霉深部感染的早期诊断,与血清GM测定联合应用诊断意义更大。
Objective To evaluate the nested PCR for early etiological diagnosis of invasive aspergillosis.Methods Detection of sera samples from the established IA model,and compared with blood culture and imaging results;comparison with the nested PCR and serum galactomannan results for the clinical diagnosis of IA.Results The positive rate of nested PCR for the rabbits sera was 80%（16/20）,and with the earliest detection at day two after infection;blood cultures of all the rabbits were negative for A.fumigatus,although with a later prognostication of infection for imaging.The sensitivity and specificity of nested PCR for the clinical samples were 64% and 100%,respectively.Conclusion With good sensitivity and specificity,the nested PCR is available for the diagnosis of IA,especially when combined with the serum galactomannan detection.
出处
《中国实验诊断学》
北大核心
2011年第1期62-65,共4页
Chinese Journal of Laboratory Diagnosis
基金
江苏省科技支撑计划社会发展专项基金(BE2009673)
