摘要
利用黑曲霉、米曲霉、康氏木霉、短乳杆菌和大肠杆菌体外转化獐牙菜苦苷。色谱分析结果表明,黑曲霉、米曲霉、康氏木霉能有效地转化獐牙菜苦苷,其中以黑曲霉的转化能力最强,转化率达到36%;短乳杆菌和大肠杆菌没有可见的转化反应。从黑曲霉转化液中分离得到了两个转化产物,分别为红百金花内酯和(Z)-5-Ethylidene-8-hydroxy-3,4,5,6,7,8-hexahydro-1H-pyrano[3,4-c]pyridin-1-one。
Swertiamarin was in vitro catalyzed by Aspergillus niger,Aspergillus oryzae,Trichoderma koningii,Lactobacillus brevis and Escherichia coli.The results showed that only A.niger,A.oryzae,T.koningii could biotransform swertiamarin.Metabolites were not observed in the cultures of L.brevis and E.coli.And the highest transformation rate（36%）of swertiamarin in culture of A.niger was obtained.Two metabolites were separated and identified as erythrocentaurin and（Z）-5-ethylidene-8-hydroxy-3,4,5,6,7,8-hexahydro-1H-pyrano[3,4-c]pyridin-1-one.
出处
《化学与生物工程》
CAS
2011年第1期33-36,共4页
Chemistry & Bioengineering
关键词
黑曲霉
獐牙菜苦苷
生物转化
分离
Aspergillus niger
swertiamarin
biotransformation
separation
