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佛波酯及3-甲基胆蒽对Bhas 42细胞系的诱癌和促癌作用 被引量：2

Initiating and Promoting Activity of TPA and MCA for Bhas 42 Cells
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摘要 根据化学致癌物在肿瘤形成过程中作用的不同阶段,可将化学致癌物分为诱癌化合物和促癌化合物,化学致癌物可使得Bhas细胞系产生突变从而形成集落。本研究通过考察促癌化合物佛波酯(TPA)及诱癌化合物3-甲基胆蒽(MCA)对Bhas 42细胞系的转化作用,验证Bhas 42细胞转化试验检验诱癌物及促癌物的准确性。 The initiator and promoter of carcinogenesis can induce foci in Bhas 42 cell lines. The initiating or promoting activity of 12-O-tetradecanoylphorbol-13-acetate （TPA） and 3-methylcholanthrene （MCA） was further confirmed by cell transformation assay using Bhas 42 cells. The assay results indicated that Bhas 42 cell line was sensitive to either initiator or promoter of carcinogenesis.
作者 宋征 刘全海 马璟
机构地区 上海医药工业研究院国家上海新药安全评价研究中心 上海医药工业研究院
出处 《中国医药工业杂志》 CAS CSCD 北大核心 2011年第1期42-44,共3页 Chinese Journal of Pharmaceuticals
基金 "十一五"重大新药创制科技重大号项(2008ZX09305-006)
关键词 细胞转化 Bhas 42细胞系 诱癌 促癌 cell transformation Bhas 42 cell line initiation promotion
分类号 R961 [医药卫生—药理学]
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参考文献8

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同被引文献26

  • 1杜艳,胡俊,刘全俊,白云飞,葛芹玉,陈宝安,吴平平,黄培林.应用多重RT-PCR技术检测食管癌患者外周血多种肿瘤基因标志物的研究[J].现代生物医学进展,2007,7(7):1007-1009. 被引量：2
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  • 7Ohmori K, Umeda M, Tanaka N, Takagi H, Yoshimura I, Sasaki K, et al. An inter-laboratory collaborative study by the Non-Genotoxic Carcinogen Study Group in Japan, on a cell transformation assay for tumour promot- ers using Bhas 42 cells [ J ]. Altern Lab Anim, 2005, 33 (6) :619-639.
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  • 9Yamasaki H. Reversible inhibition of cell differentiation by phorbol esters as a possible mechanism of the promo- tion step in chemical carcinogenesis [ J ]. IARC SciPubl, 1980, (27):91-111.
  • 10Maeshima H, Ohno K, Tanaka-Azuma Y, Nakano S, Yamada T. Identification of tumor promotion marker genes for predicting tumor promoting potential of chemi- cals in BALB/c 3T3 cells[J]. Toxicol In Vitro, 2009, 23(1) :148-157.

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中国医药工业杂志
2011年 第1期

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