摘要
目的研究实时荧光定量PCR定量检测丙型肝炎病毒(HCV)RNA载量与HCV核心抗原和丙氨酸氨基转移酶(ALT)检测的相关性及符合性。方法逆转录-PCR荧光探针法定量检测94例怀疑HCV感染患者的血清HCV RNA,同时用酶联免疫吸附试验(ELISA)检测HCV核心抗原,全自动生化分析仪检测ALT水平。结果 94份样本中HCV RNA阳性率为56.4%(53/94),核心抗原阳性率为53.2%(50/94),经统计学分析,两种方法的阳性率差异无统计学意义(P>0.05),符合率为84.9%(45/53);ALT异常率为62.3%(33/53),并随HCV RNA载量的升高而增加。结论 HCV RNA定量检测及HCV核心抗原检测结合ALT结果分析有助于临床了解HCV在体内的复制水平和肝脏的炎性反应状态,指导临床用药及观察疗效。
Objective To investigate the relevance and compliance of quantitative detection of HCV RNA by real-time fluorescence PCR and detection of HCV core antigen and alanine minotransferase.Methods HCV RNA,HCV core antigen and ALT in serum of 94 patients with suspected HCV infection were detected separately by RT-PCR fluorescent probe assay,enzyme-linked immunosorbent assay and automatic biochemical analyzer.Results The positive rates of HCV RNA and HCV core antigen were 56.9%(53/94) and 53.2%(50/94) respectively.There were no significant statistical difference between the two methods(P0.05) and the coincidental rate was 84.9%(45/53).The abnormal rate of ALT was 62.3%(33/53),which was increased with the increase of the content of HCV RNA.Conclusion Quantitative detection of HCV RNA and HCV core antigen test with ALT results can help clinicians to understand the level of HCV replication of the body and the inflammatory response status of the liver in order to guide clinical treatment and observation effects.
出处
《检验医学与临床》
CAS
2011年第2期162-163,共2页
Laboratory Medicine and Clinic