摘要
目的:建立蒙药那如三味丸中没食子酸的含量测定方法。方法:采用高效液相色谱法,Hypersil ODS C18(250mm×4.6mm,5μm)色谱柱;以甲醇-0.1%磷酸(4∶96)为流动相;流速:1.0ml.min-1;柱温为28℃;检测波长:273nm。结果:没食子酸在0.0052μg^0.0260μg范围内与其相应的峰面积值具有良好的线性关系。没食子酸的平均回收率为98.72%,RSD为1.93(n=6)。结论:该方法精密度高,分离度好,可用于蒙药那如三味丸中没食子酸的含量测定。
Objective： To establish it as the shamisen pills Mongolian gallic acid method. Methods： HPLC, Hypersil ODS C18 （250mm×4.6mm,5μm） column; methanol - 0. 1% phosphoric acid （four ninety - six） as mobile phase; flow rate： 1.0ml·min-1 ; column temperature 28℃ ; detection wavelength： 273nm. Results： gallic acid in 0. 005 2μg-0. 026 0μg range of values of their corresponding peak area has a good linear relationship. Gallic acid, the average recovery was 98. 72%, RSD was 1.93 （n = 6） . Conclusion： The method of high precision, resolution, and can be used, such as the shamisen Mongolian pill that gallic acid content.
出处
《中国民族民间医药》
2011年第1期11-12,共2页
Chinese Journal of Ethnomedicine and Ethnopharmacy
关键词
蒙药
那如三味丸
高效液相色谱法
没食子酸
That pill, such as the shamisen
performance liquid chromatography
gallic acid
