棉花抗逆相关基因GhDr1的克隆及生物信息学分析

Cloning and Bioinformatics Analysis of Cotton Gene GhDr1 Related to Resistance to Abiotic Stresses

通过TAIL-PCR的方法从棉花中克隆到未知功能的新基因GhDr1,生物信息学分析结果表明,GhDr1的预测蛋白含有酪氨酸激酶和蛋白酶C磷酸化位点、潜在的锌指类功能模块、MAPK磷酸化位点及MAPK相互作用识别位点;与GhDr1同源基因位于同一基因簇的基因多为参与逆境信号转导的蛋白。推测GhDr1基因可能在逆境信号转导途径的磷酸化级联反应中被调节,参与棉花逆境应答的生理过程。

We cloned a new cotton gene GhDr1 of unknown function by the method of TAIL-PCR.The bioinformatics analysis results were as follows：phosphorylation sites of tyrosine kinase and protein C exist in the predicted protein sequence of GhDr1;potential zinc-finger functional motif,MAPK phosphorylation sites and MAPK interaction docking motif are also found in the predicted protein sequence of GhDr1;the genes located in the same gene cluster with the homologous genes of GhDr1 are mostly involved in signal transduction pathways of abiotic stresses.So we speculated GhDr1 may be regulated in the phosphorylation cascade of signal transduction pathways and participate in the physiological process in response to abiotic stresses of cotton.