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白地霉脂肪酶在毕赤酵母表面展示 被引量：2

Display of Geotrichum candidum Lipase on Cells Surface of Pichia pastoris
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摘要 将白地霉脂肪酶基因N端与酿酒酵母FLO絮凝结构域序列融合,构建成脂肪酶毕赤酵母表面展示载体并转化毕赤酵母GS115。免疫荧光检测证实脂肪酶已展示于毕赤酵母细胞表面。甲醇诱导96 h后展示酶活性达到81 U/g干细胞,酶的热稳定性较游离酶有较大提高,50℃孵育4 h后酶活仍保持初始酶活70%以上。 P.pastoris surface display vector of lipase was constructed by fusing the N terminus of Geotrichum candidum lipase gene with FLO-flocculation domain sequence from Saccharomyces cerevisiae.The recombinant vector was then transformed into P.pastoris GS115.The fluorescence microscopy of immunolabeled P.pastoris revealed the lipase was displayed on the cell surface.The hydrolysis activity of lipase displayed on P.pastoris cell surface reached 81 U/g dry cell after 96 h of induction.The thermal stability of displayed lipase was improved compared with that of free lipase,and the residual activity was above 70 percent of initial activity after incubation at 50℃ for 4 h.
作者 陶站华 张搏
机构地区 广西科学院生物物理实验室
出处 《生物技术通报》 CAS CSCD 北大核心 2011年第1期174-178,共5页 Biotechnology Bulletin
基金 广西自然科学基金项目(桂科自0991078 桂科攻0895003-4-1) 广西科学院基本业务费资助项目(08YJ16WL02)
关键词 表面展示 毕赤酵母 脂肪酶 白地霉 全细胞催化剂 Surface display Pichia pastoris Lipase Geotrichum candidum Whole cell catalyst
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献8

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二级参考文献43

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共引文献3

同被引文献21

  • 1贾艳萍,魏群,赵军.对酵母细胞酶法破壁的研究[J].中国酿造,2005,24(9):11-13. 被引量：34
  • 2刘文山,闫云君.脂肪酶表面展示技术[J].中国生物工程杂志,2007,27(9):97-102. 被引量：4
  • 3Saleem M,Brim H,Hussain S,et al.Perspectives onmicrobial cell surface display in bioremediation[J].Biotechnol Adv,2008,26(2):151-161.
  • 4Pepper L、R,Cho Y、K,Boder E、T,et al.A decadeof yeast surface display technology:where are wenow?[J].Comb Chem High Throughput Screen,2008,11(2):127-134.
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  • 6Takayama K,Suye S,Kuroda K,et al.Surface dis-play of organophosphorus hydrolase on Saccharomy-ces cerevisiae[J].Biotechnol Prog,2006,22(4):939-943.
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  • 10Su G D,Zhang X,Lin Y.Surface display of active lipase in Pichia pastoris using Sed 1as an anchor protein[J].Biotechnol Lett,2010,32:1131-1136.

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生物技术通报
2011年 第1期

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