柿叶黄酮化合物分离制备

Separation,Purification and Structural Analysis of Flavonoids from Diospyros kaki Leaves

应用高速逆流色谱法和制备型高效液相色谱法分离制备柿叶黄酮化合物并进行初步结构鉴定。采用乙酸乙酯-甲醇-水(5:1:5,V/V)做溶剂系统,在主机转速850r/min、流速1.5mL/min、检测波长254nm条件下进行HSCCC分离纯化,所得流分浓缩干燥后再采用制备型HPLC分离制备柿叶黄酮化合物,所得产物采用HPLC检测并进行显色反应、紫外图谱和质谱分析。结果表明,从柿叶粗提物中分离所得4种化合物是以槲皮素和山奈酚为苷元的黄酮苷类化合物。

In this study,high-speed countercurrent chromatography（HSCCC）followed by preparative high performance liquid chromatography（HPLC）was used to separate and purify flavonoid monomers from the crude ethyl acetate extract from Diospyros kaki leaves.Meanwhile,the flavonoid monomers obtained were preliminarily identified by color development reaction and further structurally characterized by UV spectroscopy and mass spectroscopy.The HSCCC separation of flavonoids was achieved by using a solvent system composed of ethylacetate,methanol and water（5：1：5,V/V）at a flow rate of 1.5 mL/min and setting the rotational speed at 850 r/min with a detection wavelength set at 254 nm.For further separation,the separated fractions were then applied to preparative HPLC,and 4 flavonoid compounds,named AⅡ-,A-Ⅲ,BⅡ-and B-Ⅲ,were obtained.A-II and A-III might be quercetin flavonoids,and B-Ⅱand B-Ⅲmight be kaemoferol.