海藻胶寡糖的分离制备及电喷雾质谱分析

Enzymatic Preparation and Electrospray Ionization Mass Spectrometry(ESI-MS)Analysis of Alginate Oligosaccharides

为获得不同聚合度的海藻胶寡糖并对其进行确证,以海藻胶经酶解制得的寡糖产品为原料,对其总糖含量、糖醛酸含量以及单糖组成等理化指标进行测定,运用硅胶柱层析对寡糖混合物进行分离,以薄层色谱和电喷雾质谱对所得的寡糖单体进行分析。结果表明:酶解所得到的原料主要为聚合度2～10的寡居糖醛酸混合物;经硅胶柱层析分离可获得聚合度2～5糖的单体。本方法可分离、制备和鉴定海藻胶寡糖。

The enzymatic preparation of alginate oligosaccharides with different degrees of plormerization（DPs）from algin was achieved by using 1805 enzyme,an enzyme obtained from the 1805 group of Dalian Institute of Chemical Physics,Chinese Academy of Sciences.Hydrolyzed algin was analyzed for its contents of total sugar and uronic acid and monosaccharide composition,and meanwhile UV spectrometrical analysis at 280 nm was performed to see whether protein is present in it.In addition,the mixture of oligosaccharides was separated by silica column chromatography,and the separated fractions were analyzed by thin layer chromatography（TLC）and electrospray ionization mass spectrometry（ESI-MS）.The hydrolysis products of algin were a mixture of oligosaccharides mainly with a DP of 2-10.Monomers consisting of 2-5 glucose units were substantially separated by silica column chromatography.These investigations will provide some useful references for the preparation,separation and identification of alginate oligosaccharides.