摘要
建立一种新的、同时测定葡萄酒中14种单体酚含量的高效液相色谱法。采用ZORBAXSB-C18色谱柱(4.6mm×250mm,5μm),乙腈-冰醋酸-水溶液为流动相,梯度洗脱,流速为1.0mL/min,柱温30℃,检测波长280nm。测定6种不同地区、不同年份的赤霞珠干红葡萄酒中14种单体酚的含量。结果表明,不同地区相同品种葡萄酒中没食子酸、儿茶素、表儿茶素3种单体酚含量均较高,但14种单体酚含量各不相同,同地区不同年份的赤霞珠葡萄酒单体酚含量亦有差别,葡萄酒中几种主要单体酚随时间的变化含量均有降低,同时伴随着部分单体酚含量的增加。
The contents of 14 mono-phenols in six Cabernet Sauvignon dry red wines with different aging times or different geographical origins of grape were determined using a HPLC method.The chromatographic separation was performed on a ZORBAX SB-C18 column at 30℃with an acetonitrile/acetic acid/water mixture as a mobile phase at a flow rate of 1.0 mL/min and the detection wavelength was set at 280 nm.The contents of 14 mono-phenols in Cabernet Sauvignon wine varied with geographical origin of grape,and higher contents of gallic acid,catechin,and epicatechin were found in all the six wine samples.Cabernet Sauvignon wines with different aging times from the same region differed in their mono-phenols content as well.The contents of several major mono-phenols decreased with increasing aging time,while a few others exhibited an opposite change.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第2期180-183,共4页
Food Science
基金
2008年陕西省科技计划项目(2008XH4-2)
