春兰菌根真菌CL-3菌株的鉴定及共生体系的构建

A mycorrhizal fungi CL-3 from Cymbidium goeringii:characterization and establishment of symbiotic associations

采用分离自野生春兰(Cymbidium goeringii)根部的真菌CL-3菌株,进行了春兰内生菌根真菌的人工接种、再分离及其共生培养研究。通过对CL-3菌株形态学观察和ITS序列同源性分析,发现该菌株ITS序列与Acremonium strictum的亲缘关系最为接近,序列同源性为100%。用CL-3菌株接种春兰组培苗,接种后2个月可从组培苗中再分离获得该菌株,且CL-3菌株处理苗的鲜重增长率达80.5%,经方差分析,与对照相比有显著差异。通过石蜡切片和染色,在已接种的组培苗的根部组织中可观察到CL-3菌株存在,表明CL-3菌株能与组培幼苗成功建立共生培养体系。

The fungal strain CL-3 was isolated from the root of wild Cymbidium goeringii with using conventional isolation method.The inoculum of strain CL-3 was used to study its interaction with its host plant.According to the morphological features examination and rDNA ITS sequence analysis,the closest relative of the strain was Acremonium strictum,with the similarity of 100%.After two months of symbioses culture,the fresh biomass of C.goeringii inoculated with CL-3 increased by 80.5%,and the endophytic CL-3 was re-isolated from the seedlings.The statistics showed that the difference between inoculation group and control group was significant.The presence of the fungus in the tissues was studied with paraffin-cut section.The results showed that the fungus was observed in inner tissues of roots,indicating that the symbiosis relationship between Cymbidium goeringii and fungi could be established.