金荞麦和苦荞麦抗菌活性内生真菌的筛选及鉴定

Isolation and identification of endophytic fungi with antimicrobial activity from Fagopyrum dibotrys and Fagopyrum tataricum

从药用植物金荞麦和苦荞麦的根、茎、叶、花中分离到62株内生真菌,并以金黄色葡萄球菌(Staphylococcus aureus)、大肠杆菌(Escherichia coli)、枯草芽孢杆菌(Bacillus subtilis)[CMCC(B)63501]、小麦赤霉病菌(Fusarium graminearum)、黄瓜枯萎病菌(Fusarium oxysporum f.sp.cu-cumerinum)和绵腐病菌(Pythium aphanidermatum)6种微生物为指示菌对分离到的内生真菌进行抗菌活性检测。结果发现,分离的内生真菌菌株KQH-01、KQH-02和JQY-1的发酵醇提取物具有较好的抑菌活性。形态学特征和分子鉴定确定菌株KQH-01为炭角菌属(Xylaria sp.)真菌,菌株KQH-02为球毛壳菌(Chaetomium globosum),菌株JQY-1为葡萄座腔菌(Botryosphaeria dothidea)。

A total of 62 endophytic fungi were isolated from the plant tissues（root,stem,leaf and flower） of medicinal plants Fagopyrum dibotrys and Fagopyrum tataricum.The antimicrobial activity from isolated endophytic fungi were detected using agar diffusion method with Staphylococcus aureus,Escherichia coli,Bacillus subtilis [CMCC（B） 63501],Fusarium graminearum,Fusarium oxysporum f.sp.cucumerinum and Pythium aphanidermatum as indicator microorganisms.The results showed that the ethanol extracts of three isolated entophytic fungi strain KQH-01,KQH-02 and JQY-1 had relatively strong antibiotic activity to some indicator microorganisms.By using morphological and molecular identification,strain KQH-01was determined to belong to the genus Xylaria sp.,and strains KQH-02 and JQY-1 were identified as Chaetomium globosum and Botryosphaeria dothidea respectively.