酶法生产共轭亚油酸的研究进展

Development of linoleate isomerase catalyzes for production of conjugated linoleic acid

共轭亚油酸(Conjugated linoleic acid,CLA)具有抗癌、抗动脉粥样硬化、减肥和免疫调节等生理活性。共轭亚油酸可以通过酶法异构化获得,将底物亚油酸异构形成具有生物活性物质-共轭亚油酸的异构酶称为亚油酸异构酶。因此,通过介绍亚油酸异构酶的来源、作用机制、酶学性质和基因工程菌生产等方面的研究进展,结合不断发展的基因工程技术,旨在提高亚油酸异构酶的活性、产量和异构化效率,以扩大反应底物范围,降低生产成本,从而推进共轭亚油酸的规模化、可持续性的工业生产。

CLA（Conjugated linoleic acid,CLA） possesses a wide range of biological activities including anti-cancer activity,anti-atherosclerosis activity,capability of helping reduce weight fat and regulate immune system.The fatty acid isomerase from bacteria,which catalyzes the isomerization of linoleic acid（LA） to CLA production,is a promising candidate for other approachs.This paper introduces the source of linoleate isomerase,the mechanism of its function as well as reviewing recent advances regarding the key property of this important enzyme.The preparation of linoleate isomerase and how to use it to produce high yield of isomers of conjugated linoleic acid with high purity are also demonstrated,which will help to realize sustainability and industrial scale production of conjugated linoleic acid.