一种快速同步检测水稻黑条矮缩病毒和南方水稻黑条矮缩病毒的方法

A Simplified Method for Quick Detection of Rice Black-Streaked Dwarf Virus and Southern Rice Black-Streaked Dwarf Virus

水稻黑条矮缩病毒和南方水稻黑条矮缩病毒是近年来在水稻上危害日益严重的两种病毒,由于两者同属于呼肠孤病毒科斐济病毒属,在症状、粒体形状、传播介体及寄主等方面非常相似,由此也给诊断及鉴定造成了困难。针对这一问题,通过设计简并引物,并优化体系,建立了一种快速、简便、灵敏、特异的方法,为这两种病毒的检测及鉴别提供了方便。

Rice black-streaked dwarf virus and southern rice black-streaked dwarf virus are two important viruses in rice,which cause great losses in local rice production in recent years.The two viruses belong to Fijivirus.They share many similar characters,such as symptoms,virus particles,vectors,host range etc.,causing difficulty in discrimination of the two viruses.Three degenerate primers were designed and a simple,rapid,efficient method was described.