单、双价转基因抗虫棉对棉铃虫适合度及其抗药性的影响

Effects of Univalent Transgenic Cotton CCRI 41 and Bivalent Transgenic Cotton CCRI 44 on Fitness and Resistance to Cry1Ac of Helicoverpa armigera

室内以分别用转双价基因棉中棉所41(Cry1Ac+CpTI)、转单价基因棉中棉所44(Cry1Ac)和常规棉中棉所49(CK)连续筛选16代的AYBC、AYBT和AYCK棉铃虫种群为材料,分别继续用上述材料再连续筛选10代,研究抗虫棉对棉铃虫适合度及其抗药性的影响。试验结果表明:经室内连续筛选26代后,与AYCK对照种群相比,AYBC和AYBT种群的幼虫发育历期明显延长,蛹重、产卵量、卵孵化率均明显降低,蛹历期和雌虫百分率差异不显著;与AYBT种群相比,AYBC种群的幼虫发育历期明显延长,产卵量显著降低,蛹重、蛹历期、雌虫百分率和卵孵化率差异不显著。17-26代,AYBC种群与AYBT种群同一筛选代数间对Cry1Ac毒素的抗性倍数差异不显著,并且随着筛选代数的增加,AYBC和AYBT种群的抗性倍数都有所增加。

The Helicoverpa armigera populations AYBC,AYBT and AYCK were fed 16 generations by transgenic cotton CCRI 41,CCRI 44 and non-transgenic cotton CCRI 49,respectively.After 26 generations of selection,compared with the control population AYCK,the larval development periods of the AYBC and AYBT populations delayed;pupal weight,fecundity and egg hatching rate reduced.Compared with the AYBT population,the larval development period of AYBC population delayed,fecundity reduced.During 17th-26th generations,the resistance ratios to Cry1Ac for each generation had no difference between AYBC and AYBT populations.And with generation increasing,the resistance ratios to Cry1Ac in AYBC and AYBT populations also increased.