转几丁质酶和葡聚糖酶双价基因棉花株系对黄萎病的抗性

Verticillium Wilt Resistance of A Transgenic Cotton Line with Chitinase and Glucanase Genes

采用人工病圃、重病田及苗期室内鉴定相结合的方法,研究了转双价(Chi+Glu)基因的棉花株系61217-1对棉花黄萎病的抗性。结果表明,转基因棉花株系61217-1在人工病圃连续3年达抗病水平,病指极显著低于其受体中棉所24。2009年该转基因株系在河南安阳、江苏大丰及新疆石河子棉花黄萎病重病田的病指分别为18.7、18.1和16.7,均极显著低于受体中棉所24的病指。该转基因株系对来自河北、湖北、新疆的3个强致病力落叶型菌株均达抗病水平,病指分别为16.4、16.8和15.6,也极显著低于受体中棉所24的病指。表明转双价(Chi+Glu)基因的棉花株系61217-1具有优异稳定的抗黄萎病性。

Artificial disease nursery,severe disease field and greenhouse were jointly used for Verticillium wilt disease resistance identification of a transgenic cotton line 61217-1 into which chitinase and glucanase genes were transferred.Results showed that transgenic line 61217-1 exhibited high resistant level to Verticillium wilt disease in artificial nursery during successive three years.Average disease index of line 61217-1 was 17.9,which was significantly lower than that of the acceptor CCRI 24.In 2009,in the severe disease fields,the average disease indexes of this transgenic line were 18.7,18.1 and 16.7 in Anyang,Henan Province,Dafeng,Jiangsu Province,and Shihezi,Xinjiang,respectively.These indexes were obviously lower than those of the acceptor CCRI 24,respectively.Furthermore,this chitinase-glucanase transgenic cotton line 61217-1 also showed higher resistant to strong virulent defoliating Verticillium dahliae,which was isolated from Hebei,Hubei and Xinjiang,respectively.And their average disease indexes were 16.4,16.8 and 15.6,which were significantly lower than that of CCRI 24,respectively.The results indicated that the transgenic cotton line 61217-1（Chi＋Glu） exhibited excellent and stable resistance to Verticillium wilt disease.