卵母细胞的卵龄对猪核移植效率的影响

Effect of Recipient Porcine Oocytes on Nuclear Transfer

对核移植中，猪卵母细胞的卵龄对核移植效率的影响进行了研究结果表明：体外成熟培养 ４８ ｈ 的猪卵母细胞最适合作为核受体，其激活率达 ７８．３％ ，并无裂解；去核率高达９０％ ；在作为核受体进行核移植时，融合率为７５％ ；重构胚胎的卵裂率为６４．６％ 卵龄进一步增大，激活率略有增加，但去核率。

Studies were carried out to find out the optimal age of recipient porcine oocyte for successful nuclear transfer. The results indicated that: when oocytes cultured in vitro for 48 hours, 1. Parthenogenetical activation rate was high to 78.3% after electrostimulus; 2. Enucleation rate of oocytes was high to 90%; 3. Fusion rate and development rate of the restructured embryos was respectively 75% and 64.6%. Along with age of oocyte increase, enucleation rate of oocytes and development rate of the restructured embryos would decrease; enucleation rate of oocytes and fusion rate were no significantly change. As a result, it is recommended that oocytes cultured in vitro for 48 hours were optimal oocyte for successful nuclear transfer.