抑癌基因FHIT的克隆和序列分析

Cloning and Sequencing of Tumor suppressor Gene FHIT

用 Ｐ Ｃ Ｒ 方法，从人脑ｃ Ｄ Ｎ Ａ 文库中克隆 Ｆ Ｈ Ｉ Ｔ 基因扩增得到５５３ ｂｐ 片段克隆于ｐ Ｇ Ｅ Ｍ Ｔ 载体，测定了其 Ｄ Ｎ Ａ 序列该序列包括 Ｆ Ｈ Ｉ Ｔ ｃ Ｄ Ｎ Ａ 编码区全部４４４ ｂｐ，以及５′端５２ ｂｐ 和３′端５７ ｂｐ 非编码区序列编码区有二处位点发生突变：第９２位密码子中的 Ｃ突变成 Ｇ，是同义突变，不导致氨基酸改变；第１１２位密码子中的 Ｃ也突变成 Ｇ，导致由 Ｔｈｒ变为 Ｓｅｒ到目前为止尚未发现有 Ｆ Ｈ Ｉ Ｔ

Using PCR method, We cloned the tumor suppressor gene FHIT from human normal brain tissue cDNA library. A 553 bp FHIT gene was amplified, cloned in pGEM T vector and sequenced. The sequencing result shown this sequence included whole 444 bp nucleoside acid of FHIT encoding region as well as 5′ end 52 bp and 3′ end 57 bp noncoding region sequence. There were two point mutations in codon 92 and codon 112 where the C base was replaced by G. One mutation in codon 92 is synonymous mutation and the other in codon 112 changed it from Thr to Ser. By far we have not found similar report about site mutation leading to amino acid change in FHIT gene encoding region.