博莱霉素切割DNA反应的定量测定及其影响因素

Quantitative Determination and Effect Factor of DNA Degradation by Bleomycin

用硫代巴比妥酸反应分析了博莱霉素 Ａ５ （ Ｂ Ｌ Ｍ Ａ５ ）对 Ｄ Ｎ Ａ 的断裂作用在 Ｆｅ２＋ 和 Ｏ２存在下， Ｂ Ｌ Ｍ Ａ５能导致 Ｄ Ｎ Ａ 链断裂研究了一些因素对 Ｂ Ｌ Ｍ Ａ５ 催化 Ｄ Ｎ Ａ 断链反应的影响，设计并建立了 Ｂ Ｌ Ｍ Ａ５ 切割 Ｄ Ｎ Ａ 反应的定量测定方法，并测得 Ｋｍ 约为１５３ μｍ ｏｌ· Ｌ－ １， Ｖｍ ａｘ 约为００４６ Ａ５３２ｎｍ Ｕｎｉｔ·ｓ－ １

Cleavage action of DNA by bleomycin A 5(BLMA 5) was determined by thiobarbituric acid reaction. Breaks of DNA could be induced by BLMA 5 in the presence of Fe 2+ and O 2. Some effect factors of DNA degradation by BLMA 5 were studied. The quantitative determination method of DNA degradation by bleomycin were designed and established, and K m is 153 μmol·L -1 and V max is 0 046 A 532nm Unit·s -1 .