猪瘟病毒HCLV株E_2核苷酸序列与结构分析

Nucleotide Sequence and Structure Analysis of E 2 Gene of Hog Cholera Lapinized Virus Chinese Strain

用 Ｒ Ｔ Ｐ Ｃ Ｒ技术从 Ｃ Ｓ Ｆ Ｖ Ｈ Ｃ Ｌ Ｖ 株 Ｆ４８２ 代感染兔脾的细胞总 Ｒ Ｎ Ａ 中分段扩增出了 Ｃ Ｓ Ｆ Ｖ Ｅ２ 基因特异的３ 个部分重叠的 Ｄ Ｎ Ａ 片段（大小分别为 ４９９ ｂｐ、５７２ ｂｐ 和 ２４５ ｂｐ），并将之分别克隆到ｐ Ｇ Ｅ Ｍ Ｔ 载体上 经核苷酸序列测定和片段重叠完成了包含 Ｅ２ 全长基因的 １ １４４ ｂｐ 序列的测定，其 Ｇ Ｃ含量为４９．０％ ：核苷酸序列与国外报道的各株病毒的 Ｅ２ 基因同源性分别为８３．５％ ～９７．５％ ；其推导的氨基酸序列的同源性也分别为８９．３％～９６．２％ ，变异主要分布在 Ｅ２ 蛋白的 Ｎ

Three partly overlapped fragments encompassing CSFV E 2 gene was amplified from the total cellular RNA of the CSFV strain HCLV infected rabbit spleen by reverse transcription polymerase chain reaction(RT PCR) and was inserted into the pGEM T vector seperately. The target rejion encompassing CSFV E 2 gene was sequenced completely to be 1144bp with 49.0% of the G+C content. The nucleotide sequence of the E 2 gene of the CSFV strain HCLV was homologous to that of other strains by 83.5% ～97.5%, respectively, and the corresponding deduced amino acid sequence was homologous by 89.3% ～96.2%, respectively. The amino acid variation distributed mainly in the N half of the the glycoprotein E 2, in which the major antigenic domains located.