Tween-80对鱼腥草注射液及鱼腥草挥发油的影响

Effect of Tween 80 on Yuxingcao injection and volatile oils from Houttuynia cordata

目的:研究聚氧乙烯脱水山梨醇单油酸酯(吐温80)对鱼腥草挥发油和鱼腥草注射液的影响。方法:重点针对醛基化合物和新生成的物质,从不同产地药材、不同贮存温度、不同贮存时间的角度,对鱼腥草挥发油、鱼腥草注射液、吐温80与鱼腥草挥发油的混合溶液进行1H-NMR的测定和谱图比较。结果:4个产地鱼腥草的挥发油均存在3个醛基的1H-NMR单峰,但在与吐温80混合后这些醛基峰基本消失,并在δ8.30处出现了新的1H-NMR峰。鱼腥草注射液在醛基吸收区域未见明显的1H-NMR峰,但在δ8.30处有微弱的1H-NMR峰,该δ8.30峰在鱼腥草注射液于40℃恒温放置1到3个月后峰面积显著升高;结论:吐温80会使鱼腥草挥发油中的醛基类化合物含量显著降低并生成新的物质,鱼腥草注射液中含有微量的该新物质,并在40℃放置1个月后该新物质的含量显著升高。

Objective： To research the effect of polysorbate 80 （ Tween 80） on Yuxingcao injection and volatile oils from Houttuynia cordata. Method： ^1H-NMR spectra of aldehydic and new matter in Yuxingcao injection, volatile oils of H. cordata, and solutions of Tween 80 and volatile oil of H. cordata are determined and compared from various angles of growing origin, storage temperature, and storage time. Result： Three aldehydic singlets in ^1H-NMR spectra of every volatile oil from 4 aerial part of H, cordata were observed. These aldehydic peaks were basically disappeared and a new peak at δ 8. 30 was found in ^1H-NMR spectra of the volatile oil solutions in tween 80. Any obvious aldehydic peak in ^1H-NMR spectra did not be observed in Yuxincao injection. A weak peak at δ 8. 30 was found in ^1H-NMR spectra in Yuxincao injection, and the peak high of δ 8.30 was remarked gone up when the injection was stored in 40 ℃ for 1 to 3 months. Conclusion： Tween 80 might cause the obvious reduce of aldehydic compounds contents and the production of a novel singal at δ 8. 30 in ^1 H-NMR spectra when it was mixed with the volatile oil from the aerial part of 1t. cordata. The novel signal at δ 8. 30 in ^1H-NMR spectra existed in Yuxincao injection and was very small, but was increased remarkably when the Yuxincao injection was stored at 40 ℃ for 1 month at least.