一株新的草鱼出血病病毒分离物的特性

CHARACTERISTICS OF A NOVEL ISOLATE OF GRASS CARP HEMORRHAGIC VIRUS

从湖南邵阳地区患出血病的草鱼组织中分离到一株病毒,经部分提纯、负染后,电镜观察,揭示为双层衣壳、直径约71nm的球形颗粒。其核酸在聚丙烯酰胺凝胶电泳中呈现11条带。经核酸酶消化、吖啶橙染色及核酸的变性试验,证明为双链RNA。病毒在草鱼肾细胞株(CIK)单层细胞上,形成直径约2mm的空斑;对温度有一定稳定性;对乙醚、氯仿不敏感;经pH3、10处理后仍能保持相对稳定的滴价。此病毒不属业已建立的呼肠孤病毒科6个属中的任何一个属,而可能为呼肠孤病毒科中一新成员。

A virus isolated from hemorrhagic grass carp at Shao Yang County, Hunan Provice revealed icosahedral particles of approximately 71 nm in diameter with a double capsid under eletron microscopic observations. Polyacrylamide gel eletrophoresis showed that the viral genome was composed of 11 segments of dsRNA. This was proved by RNase or DNase treatment and acridine orange staining as well as nucleic acid denaturing assay. The virus replicated well in the CIK cell line, forming plaqus approximately 2 mm in diameter in monolayer culture. It was ether and chloroform resistant, stable at pH 3.7 and 10, and at a temperature of 56℃ for 30 min. This virus is distinct from any of the Min Jiang, Chang Jiang, Yellow River, and may be a novel member of this family.