羟基多溴联苯醚(3′-OH-BDE-7)的大鼠体外肝代谢研究

IN VITRO METABOLISM STUDY OF 3′-OH-2,4-diBDE-7 IN RAT LIVER S9 FRACTION

本文利用液相色谱-质谱联用技术,建立了羟基多溴联苯醚3′-OH-BDE-7在S.D.大鼠肝匀浆中的体外代谢研究方法.实验结果表明,3′-OH-BDE-7的主要代谢产物为2,4二-溴苯酚和二羟基多溴联苯醚(diOH-PBDEs),并且其在温孵30min,时代谢转化率为90%.3′-OH-BDE-7的代谢研究为其它羟基多溴联苯醚的相应研究提供了信息,其代谢行为也对更好地理解PBDEs和OH-PBDEs的毒理和动力学具有一定意义.

Polybrominated diphenyl ethers（PBDEs） are an important class of brominated flame retardants that have been widely used in industrial and consumer products.PBDEs have become worldwide environmental pollutants because of the bioaccumulation,long-distance transportation and potential biological toxicities.Hydroxylated polybrominated diphenyl ethers（OH-PBDEs） are a relatively new group of phenolic compounds that have attracted particular interest due to their endocrine disrupting potency.OH-PBDEs are similar to PBDEs in chemical structure,but were reported to show stronger biological effects.In this study,we investigated in vitro metabolic behaviors of selected 3′-OH-BDE-7 in rat liver S9 fraction.The obtained results indicated that 2,4-dibromophenol and diOH-PBDEs were the major metabolites of OH-PBDEs.It′s worth noting that the hydroquinone like metabolites might pose a potential health risk to human and wild animals.