槐白皮对放射损伤小鼠骨髓VEGF、G-CSF的表达及超微结构的影响

Effect of Sphora japonica on the Expression of VEGF and G-CSF in Bone Marrow Tissues and Ultra-structure of Bone Marrow in Radiation Injured Mice

目的:通过检测急性放射损伤小鼠骨髓组织中血管内皮细胞生长因子(VEGF)、粒细胞集落刺激因子(G-CSF)的表达及骨髓细胞超微结构的变化,探讨槐白皮对骨髓造血细胞和造血微环境的影响。方法:实验随机分为5组,即正常、照射对照和槐白皮高、中、低剂量(0.8、0.4、0.2mL·kg-1)组。采用免疫细胞化学SABC染色法检测放射损伤小鼠骨髓组织切片中VEGF和G-CSF的表达水平,透射电镜检测骨髓细胞超微结构的变化。结果:槐白皮高、中、低剂量组小鼠骨髓组织中VEGF的表达均显著高于照射对照组(P<0.01);槐白皮中剂量组小鼠骨髓组织中G-CSF表达显著高于照射对照组(P<0.01),但槐白皮高、低剂量组与照射对照组比较无显著性差异(P>0.05)。与正常组比较,槐白皮中剂量组小鼠骨髓组织中VEGF、G-CSF表达无显著性差异(P>0.05),槐白皮高、低剂量组表达水平低于正常组(P<0.05)。骨髓细胞超微结构显示,槐白皮治疗后其细胞排列整密,染色质均匀,无核固缩,粗面内质网较丰富,轻度扩张。结论:槐白皮能改善急性放射损伤小鼠骨髓血管微环境,增强骨髓造血细胞的恢复。

OBJECTIVE：To determine the expression of vascular endothelial growth factor（VEGF）,granulocyte colony-stimulating factor（G-CSF） and ultra-structure of bone marrow in mice with acute radiation injury,in order to evaluate the effect of Sphora japonica on the hematopoietic microenvironment and hematopoietic cells of bone marrow.METHODS：40 mice were randomly divided into 5 groups：normal group,irradiation control group,S.japonica high-dose,medium-dose and low-dose groups（0.8 mL·kg-1,0.4 mL·kg-1 and 0.2 mL·kg-1）.The expressions of VEGF and G-CSF in bone marrow tissue were determined by immunocytochemistry SABC staining.Transmission electron microscope was used to observe the change of ultra-structure of bone marrow cells.RESULTS：The expression of VEGF in bone marrow tissue in S.japonica high-dose,medium-dose and low-dose groups was significantly higher than in irradiation control group（P0.05）.The expression of G-CSF in S.japonica medium-dose group was significantly higher than in irradiation control group（P0.01）,but there was no significant difference in S.japonica high-dose and low-dose groups（P0.05）.There was no significant different in the expression of VEGF and G-CSF between S.japonica medium-dose group and normal group（P0.05）,but the expression of VEGF and G-CSF in S.japonica high-dose and low-dose groups was lower than normal group（P0.05）.The ultra-structure of bone marrow showed bone marrow cells arranged regularly and closely,chromatin was uniform,pyknotic nuclear was not found,rough endoplasmic reticulum was abundant but expanded mildly.CONCLUSIONS：S.japonica can improve the hematopoietic microenvironment in mice with acute radiation injury and promote the growth of hematopoietic cells.