摘要
目的：提取和纯化Ｓａ抗原。方法：应用ＤＥ５２离子层析法。结果：Ｓａ抗原的分子量是５０ＫＤ，存在于０．２０ｍｏｌ／Ｌ Ｎａｃｌ ＴＢＳ洗脱组分中，在－２０℃保存期为１２０天。结论：分离和纯化的Ｓａ抗原可应用于免疫印迹检测抗Ｓａ抗体。
objective: To extract and purify Sa antigen. Methods: Using DE52 ion exchang method. Result: The Sa antigen had been extracted and purified. It can be stored in - 20℃for more than 120 days. Conclusion: The Sa antigen extracted can be used in studying anti-Sa antibody.
基金
吉林省卫生厅科研基金
