地高辛掺入法检测HCV RNA含量在肝病诊断中的应用

Application of detection of HCV RNA content with digxigenionincorporated in the diagnosis of hepatic disease

目的；研究血清ＨＣＶ ＲＮＡ含量与ＨＣＶ感染者肝病程度的关系。方法：应用地高辛掺入法检测血清ＨＣＶ ＲＮＡ含量。在ＰＣＲ过程中将ＤＩＧ－ｄＵＴＰ掺入到ＰＣＲ产物叶地用ＰＣＲ ＥＬＩＳＡ法检测ＰＣＲ产物，结合标准参考样本而达定量目的。结果；丙肝后肝硬化组血清ＨＣＶ ＲＮＡ含量显著高于丙型肝炎组。

objective: To study the correlation between HCV RNA content in the serum and the degree of hepatic disease of the patients with infection of HCV.Methods: detection of HCV RNA content with dixigenionincorporated. DIG-dUTP was detected with PCR ELISA. The HCV RNA quantity was defermined with the standard sample. Result: HCV RNA content in the serum was more significantly higher in the group of liver cirrhosis after HCV than in that of hepatitis C(P <0.05). )Conclusion: The measurment of HCV RNA in the serum with digxigenion - incorporated may be used to assess the state of the patients with HCV infection.