摘要
目的：探讨乳酸杆菌对小鼠巨噬细胞免疫功能的影响。方法；采用免疫电镜，放射免疫及ＥＬＩＳＡ法，分别观察小鼠巨噬细胞的ＲＭＥ，体内外细胞毒作用及ＴＮＦ活性情况。结果：实验组小鼠巨噬细胞ＲＭＥ明显增强，巨噬细胞体内外毒性明显增高。结论：乳酸杆菌对小鼠巨噬细胞的免疫功能具有增强的调节作用，可提高巨噬细胞对肿瘤细胞的杀伤功能。
Objective: To study the immune function of Lactobacillus on macrophage of mice. Methods: Immunoelectronic microscopy, autoradiography and ELISA method were used to observe the RME, the cytotoxicity of peritoneal macrophage in vitro and in vivo and changes of TNFα, respectively. Results: The RME function was enhanced markedly afer injection of Lactobacillus, the cytotoxicity of peritoreal macrophage in vitro and in vivo enhanced and the secretion of TNFαwas higher than comtrol groups ( P < 0.01). Conclusion: Lactobacillus can regulate the function of macropjhage of mice and enhance the ability of macrophage to kill the tumor cells.
关键词
乳酸杆菌
受体介导内吞
巨噬细胞
抗肿瘤
lactobacillus
RME
murine peritoneal macrophage
immunoelectronic microseopy
cytotoxicity
