神经营养因子与生长因子在体外对人视网膜神经节细胞之作用(英文)

EFFECT OF NEUROTROPHIC FACTORS AND GROWTH FACTORS ON ADULT HUMAN RETINAL GANGLION CELLS IN VITRO

目的 研究神经营养因子（ｎｅｕｒｏｔｒｏｐｈｉｃ ｆａｃｔｏｒ）与生长因子（ｇｒｏｗｔｈ ｆａｃｔｏｒ）在体外对人视网膜神经节细胞（ｒｅｔｉｎａｌｇａｎｇｌｉｏｎ ｃｅｌｌ，ＲＧＣ）生存的作用。 方法 从捐献的尸体眼球分离并培养ＲＧＣ。ＲＧＣ之鉴定系根据形态学与免疫细胞化学研究。将各种神经营养因子与生长因子分别加入培养液。对ＲＧＣ计数，并与未加任何因子的对照组相比较。 结果 未加任何因子的培养中不见或仅有极少量的ＲＧＣ。加有神经营养蛋白３（ｎｅｕｒｏｔｒｏｐｈｉｎ３，ＮＴ３）、神经生长因子（ｎｅｒｖｅｇｒｏｗｔｈ ｆａｃｔｏｒ，ＮＧＦ）、表皮生长因子（ｅｐｉｄｅｒｍ ａｌｇｒｏｗｔｈ ｆａｃｔｏｒ，ＥＧＦ）与血小板源性生长因子（ｐｌａｔｅｄｅｒｉｖｅｄ ｇｒｏｗｔｈ ｆａｃｔｏｒ，作者单位：纽约医学院，纽约眼耳医院眼科、病理科、组织培养中心ＰＤＧＦ）的培养亦同。加有下列因子的培养中有较多的ＲＧＣ出现，计数为（细胞数／每１０ 个显微镜野）：脑源性神经营养因子（ｂｒａｉｎｄｅｒｉｖｅｄ ｎｅｕｒｏｔｒｏｐｈｉｃ ｆａｃｔｏｒ，ＢＤＮＦ）：４０８；睫状神经营养因子（ｃｉｌｉａｒｙ ｎｅｕｒｏｔｒｏｐｈｉｃ

Objective To study the effects of several neurotrophic factors and growth factors on the survival of human retinal ganglion cells(RGC)in vitro. Methods RGC were isolated from donor eyes and cultured.RGC in cell culture were identified by morphologic criteria and immunocytochemical staining.Various neurotrophic factors and growth factors were added individually to the cultures.Numbers of RGC in wells in which these agents had been added were compared with those from control wells(cultures without supplements). Results No or very few RGC were present in cell cultures containing medium without supplements or those supplemented with neurotrophin 3(NT 3),nerve growth factor (NGF),epidermal growth factor(EGF)amd platelet derived growth factor(PDGF).Numbers of RGC(per 10 fields)in cell cultures containing brain derived neurotrophic factor(BDNF),ciliary neurotrophic factor(CNTF),neurotrophin 4/5(NT 4/5)and basic fibroblast growth factor(bFGF)wer 4.08,1.23,2.63 and 2.65,respectively,significantly more than found in the control cultures. Conclusions BDNF,NT 4/5,bFGF,CNTF improve survival of human RGC in vitro,while NGF,NT 3,EGF and PDGF do not.