不同部位眼相关免疫偏离的诱导和维持

Induction and maintenance of immune deviation in different intraocular compartment

目的 观察不同种系动物眼内腔针对可溶性抗原刺激是否具有支持诱导免疫偏离的能力及其维持时间。 方法 使用牛血清白蛋白（ｂｏｖｉｎｅｓｅｒｕｍ ａｌｂｕｍ ｉｎ，ＢＳＡ）作为可溶性抗原，将其分别接种于不同动物（大鼠、兔和猴）的前房、玻璃体腔和视网膜下腔。皮下注射ＢＳＡ和完全弗氏佐剂免疫动物。皮内注射抗原观察眼内腔接种ＢＳＡ动物的迟发型超敏反应（ｄｅｌａｙｅｄｔｙｐｅｈｙｐｅｒｓｅｎｓｉｔｉｖｉｔｙ，ＤＴＨ）。定时评估免疫偏离的维持时间。 结果 所有眼内腔接种抗原的动物均显示ＤＴＨ阴性反应。不同部位抗原接种后免疫偏离的平均维持时间分别为：前房组：大鼠７０ 天，兔９０天，猴３２０ 天；玻璃体腔组：大鼠１００ 天，兔１５０ 天，猴３６０天；视网膜下腔组：大鼠５０天，兔７０天，猴３００天。 结论 不同种系动物和不同眼内腔免疫偏离的维持时间不同。生物进化与免疫系统的进化具有同步性。

Objective To determine whether the vitreous cavity and the subretinal space of different species animals support the induction of immune deviation to soluble antigen and to observe its maintenance time. Methods Bovine serum albumin(BSA)was used as a soluble antigen and inoculated into the anterior chamber(AC),the vitreous cavity(VC)and subretinal space of different animals(rat,rabbit and monkey)respectively.Recipient animals were immunized with BSA and complete Freunds adjuvant.Delayed type hypersensitivity(DTH)was assessed by skin challenge.The maintenance time of deviant immune response was evaluated in the fixed time interval. Results All the animals receiving intraocular antigen inoculation did not display DTH reaction.The maintenance time of immune deviation after inoculation of antigen in different sites is (1)anterior chamber:70 days in rat,90 days in rabbit,320 days in monkey;(2)vitreous cavity:100 days in rat,150 days in rabbit,360 days in monkey;(3)subretinal space:50 days in rat,70 days in rabbit,300 days in monkey. Conclusions The maintenance time of immune deviation varied with different species of animals and different intraocular compartment.The evolution of organisms is synchronous with that of immune system.