山莨菪碱对内毒素刺激人脐静脉内皮细胞内皮素分泌的影响

The Action of Anisodamine on ET Synthesis of Human Umbilical Vein Endothelial Cells Stimulated by Endotoxin

目的探讨山莨菪碱对内毒素刺激的人脐静脉内皮细胞内皮素分泌的影响。方法应用体外培养法及放免测定法，观察内毒素对人脐静脉内皮细胞分泌ＥＴ的影响及山莨菪碱（６５４－２）对内毒素刺激的ＥＴ合成的影响。结果（１）与对照组比较，内毒素在１～２０μｇ／ｍｌ时能刺激ＥＴ释放，１０μｇ／ｍｌ时达高峰，２０μｇ／ｍｌ时反而有所下降。（２） １０μｇ／ｍｌ内毒素作用于人脐静脉内皮细胞 ６ｈ内增加不明显（Ｐ＞０． ０５），在 １０ｈ、２０ｈ及 ３０ｈ时明显增加（Ｐ均<０．００１）。（３） ０．０２ｍｇ／ｍｌ山莨菪碱对内毒素（１０μｇ／ｍｌ）刺激ＥＴ升高抑制不明显（Ｐ＞０.０５），而０.１ｍｇ／ｍｌ及０.５ｍｇ／ｍｌ时抑制明显（Ｐ均<０.００１）。（４）与对照组比较，山茛菪碱（０.５ｍｇ／ｍｌ）对 １０μｇ／ｍｌ内毒素刺激的 ＥＴ释放在 ６ｈ内抑制不明显（Ｐ>０． ０５），而在１０ｈ、２０ｈ及３０ｈ时抑制明显（Ｐ分别＜０．０５、<０．００１和<０．００１）。结论内毒素能刺激人脐静脉内皮细胞ＥＴ的释放，这种刺激作用呈时间及剂量相关性变化，山莨菪碱能抑制内毒素刺激的ＥＴ的升高，其机制有待进一步研究。

Objective To investigate the action of anisodamine on ET synthesis of human umbilical vein endothelial cells stimulated by endotoxin. Methods Human umbilical vein endothelial cells (HUVEC) were isolated and cultured,the effect of endotoxin on the ET synthesis of HUVEC,and the possible action of anisodamine were observed with RIA.Results (1) The effect of endotoxin on ET release from HUVEC was significant between the different concertrations of 1μg/ml--20μg/ml,as compared with the control.The maximum stimulation for ET release was obtained with 10μg/ml endotoxin, there was a slight reduction of ET release at the concentration of 20μg/ml,(2) The effect of endotoxin on ET release from HUVEC in different incubation time showed no significant increase in 6hrs,but significant in 10hrs, 20hrs and 30hrs as compared with that of the control. (3) The effect of anisodamine of different concentrations on HUVEC ET release stimulated by 10μg/ml endotoxin revealed that the depression of ET release wasn't significant at 0. 02mg/ml anisodamine (P>0.05),but was significant at 0. 1mg/ml and 0'.5mg/ml (P<0.001). (4) In different incubation time the effect of 0.5mg/ml anisodamine on HUVEC ET release stimulated by 10μg/ml endotoxin showed the depression of ET wasn't significant in 6hrs,but was significant in 10hrs.20hrs and 30hrs. Conclusion Endotoxin can stimulate HUVEC to release ET,the effect is dependent on time and dose.Anisodamine can influence ET release in HUVEC,its mechanism needs to be further investigated.