东南太平洋智利竹鱼RAPD遗传多样性研究

Genetic diversity of Chile jack mackerel (Trachurus murphyi) in the southeast Pacific by RAPD analysis

根据2009年7-12月我国大型拖网渔船在东南太平洋海域(34°S～37°S、90°W～110°W)作业期间采集的样本,选取17个站点146尾智利竹鱼肌肉样本进行随机扩增多态性DNA分析。结果显示扩增条带中共检测到52个位点,其中多态性位点为51个,多态位点占所有扩增位点的98.08%。群体的Shannon’s多样性指数为0.520 2±0.181 3,Nei’s基因多态性指数为0.350 0±0.144 0,种群间遗传分化指数GST为0.031 1。结果表明该海域智利竹筴鱼群体的遗传多态性较高,但群体间不存在显著的遗传分化。

According to the Chile jack mackerel（Trachurus murphyi） samples in the southeast Pacific Ocean（34°S ~37°S,90°W-110°W） collected by Chinese trawler fishing vessel during July and December 2009,146 muscle samples collected from17 sites were used for population genetic structure identification by Random Amplified Polymorphic DNA（RAPD） analysis.52 loci were detected and 51 of these loci were polymorphic,which accounted for 98.08% of the total loci.The Shannon＇s index was 0.5202±0.1813,the Nei＇s genetic diversity index was 0.3500±0.1440,and the GSTvalues was 0.0311.The results indicated that the genetic diversity of the Trachurus murphyi was comparably high,and genetic differentiation was not significant among populations.