条石鲷仔稚鱼发育过程中消化酶活性的变化

The variations of digestive enzyme activities during the development of Oplegnathus fasciatus larvae

研究了条石鲷(Oplegnathus fasciatus)从初孵仔鱼到孵化后33日龄稚鱼期间的胰蛋白酶、酸性蛋白酶、淀粉酶和脂肪酶活性变化。结果显示:条石鲷初孵仔鱼体内可检测到胰蛋白酶、酸性蛋白酶、淀粉酶和脂肪酶活性,但胰蛋白酶、酸性蛋白酶和淀粉酶活力均很低。9日龄仔鱼胰蛋白酶总活力(U/larva)有显著升高,并随发育的进行逐渐升高。28日龄稚鱼酸性蛋白酶和脂肪酶的总活力有显著增大,33日龄稚鱼的蛋白酶和脂肪酶总活力较28日龄稚鱼显著增大。9日龄仔鱼的淀粉酶总活力显著升高,并随发育的进行逐渐增大,但进入稚鱼期后淀粉酶活力随稚鱼生长发育而显著降低。研究表明条石鲷仔稚鱼消化酶活性与其发育阶段及食性密切相关,在早期仔鱼阶段,胰蛋白酶和淀粉酶是起主要消化作用的内源性消化酶。

This paper investigated the trypsinase,acid protease,amylase and lipase activities of the Oplegnathus fasciatus larvae cultured in indoor pond from hatching to post hatching day（phd） 33rd.The results indicated that trypsinase,acid protease,amylase and lipase could be detected in newly hatched larvae,although the activities of trypsinase,acid protease and amylase were very low.The total activity of trypsinase（U/larva） of the larvae increased significantly on phd 9th and increased gradually with the development.The total activities of acid prolease and lipase of the larvae increased significantly on phd 28th.The total activities of acid prolease and lipase of the larvae on phd 33rd were significant higher than those on phd 28th respectively.The total activity of amylase of the larvae increased significantly on phd 9th and also increased gradually with the development.While the larvae developed at juvenile stage（phd 22nd）,the total activity of amylase decreased significantly with the development.It is suggested that the activities of digestive enzyme of the Oplegnathus fasciatus larvae closely correspond to the development stage and feeding habit.The trypsinase and amylase were the very important endogenous enzymes at the early stage of the larva development.