摘要
利用负载型尿素层析柱对共轭亚油酸甲酯异构体进行分离。考察了固定相中甲醇残留量、尿素硅胶质量比、固定相结晶温度、层析温度及洗脱剂种类对分离效果的影响。结果表明：当固定相中甲醇残留量为3％～5％，尿素硅胶质量比为1：1，固定相结晶温度0～8℃，层析温度0℃，洗脱剂为石油醚时，经过层析分离反10，顺12共轭亚油酸甲酯的含量从样品中的33．94％提高到57．69％。
The conjugated methyl linoleate isomer was separated by urea column chromatography , and the factors influencing the separation effects of conjugated methyl linoleate isomer were investigated. The resuits showed that the content of t10,c12 conjugated methyl linoleate could be improved from 33.94% to 57.69% at the followed conditions： methanol residue in fixed phase 3% -5% , mass ratio of urea to silica gel 1 ： 1, crystallization temperature 0 - 8℃operation temperature 0 ℃, eluent petroleum.
出处
《中国油脂》
CAS
CSCD
北大核心
2011年第1期9-12,共4页
China Oils and Fats
关键词
尿素柱层析
共轭亚油酸甲酯
异构体
urea column chromatography
conjugated methyl linoleate
isomer
