纳米Eu_2O_3对HEK-293T细胞的生物活性影响

Effect of Biological Activity of Nano Eu_2O_3 on HEK-293T Cells

稀土化合物的生物效应研究已经引起广泛关注.利用激光共聚焦显微镜观察到纳米Eu2O3能进入活HEK-293T细胞中且位于细胞核周围.在体外培养条件下,利用流式细胞仪法检测了纳米Eu2O3对细胞的活力及对细胞周期的影响,结果表明当纳米颗粒浓度小于200μg?mL-1时,对细胞活性没有明显影响;而当纳米颗粒浓度≥400μg?mL-1时,对细胞活性明显抑制.当Eu2O3纳米颗粒在低浓度范围(≤50μg?mL-1)时对细胞周期基本没有影响,而高浓度(≥200μg?mL-1)时对细胞周期均有显著抑制,停滞在DNA合成后期.这些研究结果均表明纳米Eu2O3对HEK-293T细胞具有明显的生物效应.

The research on the biological effect of rare earth compound has aroused wide concerns.In this paper,the results reveal that nano Eu2O3 can be internalized by live HEK-293T cells and accumulate in the perinuclear region in（Dulbecco＇s Modified Eagle Medium） DMEM with 10%（Fetal bovine serum） FBS by laser scanning confocal microscope.In vitro culture conditions,the effects of nano Eu2O3 on cell viability and the cell cycle were investigated using flow cytometry.The results showed that cell activity was free from nanoparticles with concentration less than 200 μg？mL-1.When nanoparticles concentration is more than 400 μg？mL-1,the cell activity was inhibited.Eu2O3 nanoparticles at low concentration（≤50 μg？mL-1） had no effect on the cell cycle,while high concentrations（≥200 μg？mL-1） cell cycle was significantly in-hibited,which showed DNA synthesis in the late stagnation.These results indicated that nano Eu2O3 on HEK-293T cells have obvious biological effects.