摘要
提取新疆沙冬青叶片中蛋白质受到脂类、酚类等次生代谢产物的干扰,针对这种现象,进行了组分提取优化方法和双向电泳技术的改进。实验分析表明,用乙醚淋洗完整叶片,50 mmol.L-1Tris-HCl缓冲液,上样量240μg(7 cm胶条),考马斯亮蓝染色等方法可很大程度增加提取可溶性蛋白含量,结合丙酮-三氯乙酸的沉降,可较完整地提取蛋白质组。
Proteins extraction from leaves of Ammopiptanthus nanus(M.Pop.) Cheng f.is disturbed by some secondary metabolites,such as lipoidal and phenolic substances.To overcome this problem,method of proteins extraction and two-dimensional electrophoresis protocol of Ammopiptanthus.nanus(M.Pop.)Cheng f.were optimized and ameliorated in this paper.Further,study of preparing method,quantitative loading of samples and staining means were conducted.The results show that washing whole leafs with aether,extracting soluble proteins with 50 mmol·L^-1 Tris-HCl(pH 8.5),combined with acetone-trichloroacetic acid depositing is a good way of extracting proteins from Ammopiptanthus.nanus(M.Pop.)Cheng f.
出处
《中国沙漠》
CSCD
北大核心
2011年第1期96-100,共5页
Journal of Desert Research
基金
新疆维吾尔自治区"十一五"重大科技专项(200731138-2)
新疆维吾尔自治区自然科学基金项目(200821160)
新疆生物资源基因工程重点实验室开放课题(XJDX0201-2005-09)资助
关键词
蛋白质组
提取
双向电泳
新疆沙冬青
proteome
extraction
two-dimensional electrophoresis
Ammopiptanthus.nanus(M.Pop.)Cheng f.