乳腺癌组织中细胞粘附分子CD15的定量研究

QUANTITATIVE ANALYSIS OF CELL ADHESION MOLECULE CD15 IN HUMAN BREAST CARCINOMAS

为了探讨细胞粘附分子 Ｃ Ｄ１５ 表达及含量与乳腺癌分化程度和淋巴结转移状况的关系，应用微波 Ｓ Ｐ法和图像分析技术，对９４ 例乳腺癌和 １０ 例正常乳腺组织中 Ｃ Ｄ１５ 的表达及含量进行检测。结果： Ｃ Ｄ１５ 表达阳性物质在正常乳腺中很有极性地位于腺上皮游离面，而在乳腺癌中主要分布于浆膜； Ｃ Ｄ１５ 表达阳性率和图像定量分析平均光密度值在乳腺癌组织中均显著高于正常乳腺组织（ Ｐ＜ ０．００５， Ｐ＜ ０．００１），并均随乳腺癌分化程度减低和淋巴结转移而显著增高（ Ｐ＜ ０．０５， Ｐ＜ ０．００１）。结果表明， Ｃ Ｄ１５ 表达与乳腺癌的发生、细胞增殖、分化和转移密切相关，对判断乳腺癌恶性程度、预测生物学行为和预后可能是一良好标志。

To study the relationship between the expression and contents of cell adhesion molecule CD15 and differentiation and lymph nodes metastasis of breast carcinomas, CD15 expression and its contents in 94 cases of breast carcinomas and or cases of normal breast tissue were evaluated by microwave SP immunohistochemical chenique combined with image analysis. CD15 immunoreactivity in normal breast tissue was mainly localised at the border of gland, but in breast cancer tissues it was mainly localised in the membrane and cytoplasm. Positive rate of CD15 and its average optic density in breast carcinomas were significantly higher than those in normal breast tissue ( P <0.05 and P <0.001, respectively). The worse tumors differentiated and the earlier lymph nodes metastasized, the higher CD15 expressed and its optic density was measured ( P <0.05 and P <0.001, respectively). These results suggest that CD15 expression and its contents might be a useful indicator to evaluate the malignancy and biological features, and could be considered as a good prognostic predictor for breast carcinomas.