肝组织内源性β-葡萄糖醛酸酶活性与胆红素结石的关系

STUDY ON THE RELATIONSHIP BETWEEN THE ACTIVITY OF ENDOGENOUS β GLUCURONIDASE IN HEPATIC TISSUE AND BILIRUBIN STONE

为探讨内源性 β葡萄糖醛酸酶（β Ｇ）活性与胆红素结石的关系，采用免疫组化方法，对 ４４ 例胆红素结石，８例胆固醇结石及 ２５ 例肝外伤之肝组织中 β Ｇ 活性表达进行定位定量比较研究。结果： 胆红素结石肝组织中 β Ｇ活性表达阳性反应细胞百分率（４９．２％ ±４．６％ ）明显高于胆固醇结石肝组织（３２．５％ ±３．８％ ）和外伤肝组织（２７．８％ ±４．２％ ）， Ｐ＜ ０．０５； 胆红素结石组内 β Ｇ 活性表达阳性反应细胞百分率与患者年龄、病程长短、结石大小等因素均无相关性。结论：内源性 β Ｇ活性与胆红素结石密切相关，不同个体间其活性差异可能是影响结石形成的内在因素之一。

To study the relationship between the activity of endogenous β glucuronidase (β G) in hepatic tissue and the formation of bilirubin stone. We assessed the β G activity in hepatic tissue of 44 cases of bilirubin stone, 8 cases of cholesterol stone and 25 cases of liver injury by using immunohistochemistry. Results: The cell percentage of β G positive reaction in bilirubin stone group (49.2%±4.6%) was significantly higher than those in cholesterol stone group (32.5%±3.8%) and liver injury group (27.8%±4.2%), P <0.05. There was no relationship between cell percentage of β G positive reaction and age, patient history, size of stone. Conclusions: The activity of endogenous β G is closely related to the formation of bilirubin stone. The difference of activity between individuals is possibly the intrinsic factor which may influence the formation of bilirubin stone.