过敏性紫癜患儿单个核细胞凋亡与细胞因子、IGF-1的关系探讨

Relationship between the apoptosis of mononuclear cell and the expression of cytokines and insulin-like growth factor-1 in patients with henoch-schonlein purpura

【目的】探讨过敏性紫癜(henoch-schonlein purpura,HSP)患儿单个核细胞(mononuclear cell,MNCs)凋亡的变化,并检测周围血中细胞因子白细胞介素-8、白细胞介素-6、肿瘤坏死因子-α和胰岛素样生长因子Ⅰ的变化,探讨其对MNCs凋亡的影响。【方法】采用流式细胞术检测36例HSP患儿和26名健康对照者MNCs的凋亡情况,酶联免疫吸附试验(ELISA)检测细胞因子水平。【结果】HSP患儿MNCs凋亡率明显高于健康对照组患儿,患儿周围血中所测因子水平均高于正常对照组。细胞因子、IGF-1升高的水平与MNCs的凋亡比例成正相关。【结论】HSP患儿MNCs凋亡增加。存在免疫功能紊乱,炎性细胞因子、IGF-1产生过多可能是导致MNCs凋亡增加的重要机制,通过适度调控细胞因子、IGF-1的分泌和MNCs凋亡有可能会改善HSP预后。

【Objective】 To observe the changes of mononuclear cell（MNCs） apoptosis and the level of inflammatory cytokines and IGF-1 in patients with henoch-schonlein purpura（HSP）,and to study the relationship between them. 【Methods】 The apoptosis of MNCs in 36 patients with HSP and 22 healthy controls were determined with flow cytometry.The inflammatory cytokines and theIGF-1 were tested with ELISA method. 【Results】 The percentage of MNCs apoptosis in the patients with HSP was significantly higher than that of healthy controls.The levels of IL-8,TNF-α,IL-6,IGF-1 in patients with HSP were significantly higher than those of healthy controls,and the increased levels of inflammatory cytokines and IGF-1 in HSP patients were positively correlated to the percentage of MNCs apoptosis. 【Conclusions】 The data demonstrates that there is a promote apoptotic process of MNCs in active HSP.The high expression of inflammatory cytokines and IGF-1 may play an important role of promoting MNCs apoptosis which reduces the functional life span of MNCs.For this reason,modulating the expression of cytokines,IGF-1 and moderately controlling the apoptosis of MNCs may be a new therapeutic target for HSP treatment.