正交试验优化铁棒锤生物碱提取工艺

Study on Optimization of Extraction Technology for Total Alkaloids of Aconitum flavum by Orthogonal Design Method

目的:优选铁棒锤生物碱的最佳提取工艺。方法:选择溶剂、提取次数、洗涤次数为考察因素,以总生物碱的含量为考察指标,采用正交试验法优选铁棒锤生物碱的最佳提取工艺。结果:铁棒锤生物碱的最佳提取工艺为浸提次数3次,提取溶剂为乙醚-三氯甲烷(3∶1),洗涤1次。结论:优选得到的提取工艺稳定性好、得率高、简便可行。

Objective：To optimize the extraction technology for total alkaloids in the root of Aconitum flarum. Method： Dissolvent, times of extraction and washing were the test factors and the total alkaloids were the test index. The orthogonal design was used to optimize the extraction technology of total alkaloids. Result： The optimal extraction method was as follows ： the ether ： chloroform （3 ： 1 ） as dissolvent, extraction 3 times and washing once. Conclusion： The technology after optimization had a good stability, the yield of total alkaloids was high, and the method was easy to operate.