中药水蛭提取及精制工艺的研究

Studies on Extraction and Purification Technology of Chinese Medicine Leeches

目的:研究中药水蛭的提取及精制工艺,为水蛭提取物入制剂工艺提供技术依据。方法:依正交试验法,逆流循环提取技术,以效价法检测抗凝血酶活性为指标,对水蛭提取工艺进行优化,并运用膜分离技术对提取液进行精制。结果:最佳工艺为以12倍量0.9%氯化钠溶液提取5 h,循环3次最好,选择相对分子质量为30×103和0.5×103的膜即可达到精制的目的。结论:该提取、精制工艺稳定、合理、可行。

Objective：Studies on extraction and purification technology of the Chinese medicine leeches, preparing process of extracting leeches to provide technical basis. Method：The extraction of leeches was optimized by orthogonal experiment and countercurrent circuit extractive technique, with titer detected by anti-thrombin activi- ty as an index, and using of membrane separation technology to refine extract. Result： The best technology was to extract five hours with 12 times the amount of 0.9% sodium chloride solution, and extract three times. Selected membrane with 30 ×10^3 and 0. 5 ×10^3 could achieve the purpose of the purification. Conclusion ： The extraction and purification technology was stable, reasonable and practicable.